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Process of Recombinant DNA Technology
Recombinant DNA technology involves the following steps:
Biotechnology:
Biotechnology deals with micro-organisms, plants or animal cells or may be their enzymes to produce products
Biotechnology in agriculture:
Biotechnology can be used in:
Agro-chemical-based agriculture.
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Principles of Biotechnology
The two core techniques that developed modern biotechnology are:
(i) Genetic en
Biotechnology in medicines:
The recombinant DNA technology is used in the production of therapeutic drugs and these drugs a
Separation and Isolation of DNA Fragments (Gel Electrophoresis)
Gel electrophoresis is a technique for separating DNA fragme
Tools of Recombinant DNA Technology
The key tools required for the recombinant DNA technology are:
(i) Res
FEATURES TO CLONE A VECTOR :
Transgenic animals:
Animals that have had their DNA manipulated to possess and express an extra gene are known as transgenic animals
Genetically modified organisms:
Plants, bacteria, fungi and animals whose genes have been altered by manipulation are called Genetically Mo
Bio-piracy:
Biopiracy is the term used to refer to the use of bio-resources by multinational companies and other organizatio
Ethical issues:
Genetically modified items wether beneficial or unsuitable is checked by some ethical branches.
Therefore, the
SELECTION OF RECOMBINANT
Selection of recombinants formed can be done by one of the following methods.
Cloning Vectors
The vectors are the DNA molecules that can carry a foreign DNA segment into the host cell.
Competent Host (For Transformation with Recombinant DNA)
DNA being a hydrophilic molecule, cannot pass through cell membranes.
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