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Process of Recombinant DNA Technology
Recombinant DNA technology involves the following steps:
Principles of Biotechnology
The two core techniques that developed modern biotechnology are:
(i) Genetic en
Biotechnology:
Biotechnology deals with micro-organisms, plants or animal cells or may be their enzymes to produce products
Biotechnology in agriculture:
Biotechnology can be used in:
Agro-chemical-based agriculture.
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Separation and Isolation of DNA Fragments (Gel Electrophoresis)
Gel electrophoresis is a technique for separating DNA fragme
Tools of Recombinant DNA Technology
The key tools required for the recombinant DNA technology are:
(i) Res
FEATURES TO CLONE A VECTOR :
Biotechnology in medicines:
The recombinant DNA technology is used in the production of therapeutic drugs and these drugs a
SELECTION OF RECOMBINANT
Selection of recombinants formed can be done by one of the following methods.
Cloning Vectors
The vectors are the DNA molecules that can carry a foreign DNA segment into the host cell.
Competent Host (For Transformation with Recombinant DNA)
DNA being a hydrophilic molecule, cannot pass through cell membranes.
Transgenic animals:
Animals that have had their DNA manipulated to possess and express an extra gene are known as transgenic animals
Bio-piracy:
Biopiracy is the term used to refer to the use of bio-resources by multinational companies and other organizatio
Ethical issues:
Genetically modified items wether beneficial or unsuitable is checked by some ethical branches.
Therefore, the
Genetically modified organisms:
Plants, bacteria, fungi and animals whose genes have been altered by manipulation are called Genetically Mo
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