Somatic Hybridization:

The process of fusion of protoplast of somatic cells obtained from different varieties or species of plant on a suitable nutrient medium in vitro to develop a somatic hybrid is called somatic hybridization.

It is done by following steps:

• Single cell from selected plants are selected.
• The cell wall of selected cells are digested to get the protoplast only.
• Naked protoplasm only surrounded by plasma membrane are isolated.
• The hybrid protoplasts are cultured in a suitable media to form new plants.